Interaction between phloretin and insulin: a spectroscopic study

Abstract Diabetes is among the top ten deadly diseases in world. It occurs either when pancreas does not produce enough insulin (INS) or body cannot effectively use it produces. Phloretin (PHL) has a biological effect that can treat diabetes. A spectroscopic study was carried out to explore interaction between phloretin and insulin. UV/Vis spectroscopy, fluorescence circular dichroism spectropolarimeter were used study. spectra showed PHL INS produced strong absorption at wavelength of 282 nm. The analysis results excitation emission occurred 280-nm 305-nm wavelengths, respectively. Temperature changes did affect emissions. However, PHL–INS caused redshift 305 317 affected binding constant ( K ) site n ). Ka decreased with increasing temperature increased site. thermodynamic parameters such as enthalpy (? H 0 entropy S each had value ? 16,514 kJ/mol 22.65 J/mol·K. formed hydrogen bonds hydrophobic interaction. free energy (?G recorded negative. interactions took place spontaneously. quenching dynamic static. D values greater than . higher temperature, less appearance two negative signals on (CD) implies could induce regional configuration addition revealed proportion ?-helix stabilizes its structure. Phloretin’s stabilization enhancement structural indicate improve resistance.